Coding

Part:BBa_K125810:Design

Designed by: Grace Kwan   Group: iGEM08_Hawaii   (2008-10-27)


slr2016 signal sequence + GFP fusion for secretion of GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 702


Design Notes

GFP had to be a fusion brick so that translation would continue in frame.


Source

slr2016 signal sequence was derived from Synechocystis sp. PCC 6803 while GFP fusion was modified from the BioBrick part BBa_E0040.

References